First Response Early Result Pregnancy Test, 2 Pack (Packaging & Test Design May Vary)

I got a 2 count pack, my tests were very clearly negative with no indent lines even after evaporation. If it’s real the box should be wrapped in plastic, they should have pink cap. the shape of the test should look exactly like it does on First Responses site, also make sure they aren’t expired. You’re looking for a chemical reaction that results in the color pink, grey could easily be from evaporation. if you get a second line at all try the drugstore or go to the doctor for a blood test.

My husband and I are doing IUI so I keep these around to test when needed. They are quick and work great

Great tests fast shipping and definitely real first response tests. Some reviewers said they got knock offs. I’ve ordered from the same listing several times and always received real ones. A few have had indent lines that looked pink giving me false hope but for the most part great experience.

I really like this pregnancy test. Will order more

It did it’s job I guess.. was hard to read the lines and I ended up just getting a digital test.

I decided to post some data about this FRER sensitivity for those of you who are like me wonders how the test reflects the actual HCG concentrations. I made serial dilutions of human recombinant HCG (Novarel), that I had some leftovers of after my IVF cycle. The dilutions were made with lab pipets, so the concentration should be pretty precise, and in the appropriate buffer (sorry for the details, that was my husbands urine) to model the amount of HCG in the urine for the test. Here are results (see photos attached too). At 5 mU/ml, which is below the threshold of 6 mU/ml ( the sensitivity, claimed by the brand), the test is very-very faint. However, I would consider it positive, because just husbands urine only gives no second line at all (I do not have photo for this, we did it some time ago with our first unsuccessful transfer, when I had chemical pregnancy). 5 mU/ml in your first morning urine is equal about 10-15 mU/ml in blood, so this is comparable to 5-4 days before your missed period. Also, compare the 5 mU/ml results with my actual results for 8 days post transfer of 6-day blastocyst (last picture), two days later at 10dpt I had blood beta-HCG level of 38. At 20 mU/ml we can see clear positive results. This is HCG level that you will have at your first day of missed period, and equals to about 40-60 mU/ml of beta-HCG in blood. And only at 100 mU/ml we see the second line at the same brightness as the control line. I would say at this HCG level the FRER test reaches its saturation point. Also, I found out that you may use LH tests (used for ovulation detection) instead of HCG for the early results. LH tests are very sensitive, looks like even more sensitive than FRER (you can see how much darker the test line is for the 5 mU/ml sample). I could not find an information about what subunit of LH these tests are detecting, my understanding it is beta-subunit of LH which is nearly identical in structure to beta-subunit of HCG. Beta-HCG, however, has about 30 additional amino acids, so home pregnancy tests designed to pick up beta-HCG only, while home ovulation tests will pick up both. After ovulation and during pregnancy LH levels are down, so in my opinion you can easily use you LH leftover test to monitor HCG levels, at least to see how it doubles, for example. Another observation for FRER, these tests tend to get darker with time. If your HCG levels are expected to be below or around the threshold (like day 6 post IVF transfer or so), and the test line does not show up, I suggest you keep the test for another 24 h and you may see faint positive later. For those users, who claim about false positive FRER results. As my experiments with my husbands urine proved, if you do not have HCG, the test will be clear negative - no test line at all, only control line. If you see faint positive, which lately with another test disappeared, or if your period came, that means you had so called chemical pregnancy. It might be devastating, I understand, we had a chemical with our first failed IVF cycle. But this is a drawback of using very sensitive test, so just be prepared. To summarize my experiment: FRER is very sensitive, it shows faint positive even below claimed threshold, however good positive results are at about 20 mU/ml, or your first day of missed period. I give only 4 stars for these tests because of the idiotic curved handle - it is completely unnecessary design, makes it difficult to lay flat, and takes more space in the drawer if you want to keep the used test for future reference.

Does the job in finding out what its meant to find out

Its for my girlfriend so I dont know if she use it or not. So far so good. She havent told me if shes pregnant.

I believe First response is very sensitive when it reads HCG. However, in the past year, Ive noticed that there are more indent lines than actual positive lines. Those indent lines are tricky and can lead to pre-excitement when in actuality its a negative. My biggest suggestion would be to use the test alongside the app for First response. The app will let you know when to test based on your last menstrual cycle. Happy Testing!